BLI Analysis

Biolayer Interferometry (BLI) is a label-free, optical biosensing technique that enables real-time analysis of biomolecular interactions. It measures changes in the interference pattern of white light reflected from a biosensor surface as molecules bind or dissociate, providing quantitative insights into binding events. It has many applications in antibody discovery:

• Affinity Ranking
  Rapidly compares binding strengths of multiple antibodies to a target antigen by measuring equilibrium binding responses. Enables prioritization of candidates based on relative affinity.

• Kinetics Analysis
  Determines association (kon) and dissociation (koff) rates, yielding precise affinity constants (KD). Supports mechanistic understanding of binding behavior and informs lead optimization.

• Competition Assays
  Assesses whether two molecules compete for the same binding site by sequentially exposing the biosensor to each. Useful for characterizing blocking antibodies or receptor-ligand interactions.

• Epitope Binning
  Groups antibodies based on overlapping or distinct binding epitopes using pairwise competition formats. Facilitates selection of diverse antibody panels and informs IP strategy.